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SIGMA ALDRICH Agarose 100g


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 Rp. 10,093,900
Katalog A9539-100G
Brand Sigma Aldrich
Kondisi New
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Harga dan Ketersediaan

Katalog A9539-100G
Ukuran -
Ketersedian Hubungi Kami
Harga Rp. 10,093,900
DAPATKAN PENAWARAN
BELI
Ulasan

Agarose

low EEO, for routine use

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature. 
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Application

Ideal for routine analysis and purification of nucleic acids. Exhibits low background after staining with ethidium bromide or SYBR Green.

Properties

Related Categories Agarose, Core Bioreagents, Life Science Reagents for Cloning, Life Science Reagents for DNA/RNA Electrophoresis, Life Science Reagents for Northern and Southern Blotting
form   powder
impurities   ≤10% Moisture
color   white to off-white
EEO   0.09-0.13
transition temp   gel point 36 °C ±1.5 °C (1.5% gel)
gel strength   ≥1200 g/cm2 (1% gel)
anion traces   sulfate (SO42-): ≤0.15%
foreign activity   DNase, RNase, none detected

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