| Rp. 5,679,165 |
Description Blood adan Tissue Kit
For purification of total DNA from animal blood and tissues and from cells, yeast, bacteria, or viruses
DNeasy Blood & Tissue Kits provide fast and easy silica-based DNA purification in convenient spin-column and 96-well-plate formats. Most samples can be directly lysed with proteinase K, eliminating the need for mechanical disruption and reducing hands-on time. Optimized protocols for specific sample types provide reproducible purification of high-quality DNA for life science, genotyping, and veterinary pathogen research applications. Purification of DNA using the DNeasy Blood & Tissue Kit can be automated on the QIAcube.
Genotyping of horses.
DNA was purified from hair of 3 brown horses and analyzed for coat-color genotypes using the MSH and ASIP PCR systems. [A] MSH PCR products were digested with TaqI. Horses 1 and 3 were identified as Ee heterozygotes, carrying the recessive e allele for red coat color. [B] Analysis of ASIP PCR products identify horses 1 and 2 as Aa heterozygotes, carrying the recessive a allele for black...
|Applications||PCR, real-time PCR, genotyping|
|Elution volume||100–200 µl|
|Format||96-well plate, spin column|
|Main sample type||Blood, tissue|
|Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein||DNA|
|Sample amount||100 µl/25 mg|
|Time per run or per prep||20 minutes – 1 hour|
|Yield||6 µg/30 µg|
The efficient DNeasy Blood & Tissue procedure enables high yields of total DNA from animal blood and tissue samples (see table "Typical DNA yields from animal tissues using DNeasy Blood & Tissue Kits" and figure DNA yields). Optimized protocols ensure high yields from nonstandard samples, such as animal hair (see figure Genotyping of horses), as well as cultured cells, fixed tissues, or Gram-positive and -negative bacteria. Specialized online protocols are available for DNA purification from yeast, insects, hair, and other sample types.
|Mammalian blood||100 µl||3–6|
|Bird blood||5 µl||9–40|
|HeLa cells||2 x 106||15–25|
|Mouse tail||1.2 cm (tip)||10–25|
|Rat tail||0.6 cm (tip)||20–40|
|Pig ear||25 mg||10–30|
|Horse hair||10 hairs||2–4|
|Fish fin||20 mg||10–20|
|Fish spawn (mackerel)||10 mg||5–10|
DNeasy Blood & Tissue Kits simplify purification of DNA from a wide range of sample types, including animal species commonly encountered in life science, veterinary, and genotyping applications (see figure High-quality DNA). Purified DNA is free from PCR inhibitors, enabling sensitive detection in standard, multiplex (see figure Efficient 16plex PCR), and real-time PCR (see figure Real-time PCR). DNeasy Blood & Tissue Kits provide reliable results — from laboratory analysis of transgenic mice (see figure High-throughput purification) to livestock breeding programs (see figureGenotyping of pigs) and pedigree genotyping. Routine testing applications can be easily scaled up using the DNeasy 96 Blood & Tissue Kit (see figure High-throughput purification).
DNeasy Blood & Tissue Kits are designed for rapid purification of total DNA (e.g., genomic, mitochondrial, and pathogen) from a variety of sample sources including fresh or frozen animal tissues and cells, blood, or bacteria.
The DNeasy membrane combines the binding properties of a silica-based membrane with simple microspin technology or with the QIAGEN 96-Well-Plate Centrifugation System. DNA adsorbs to the DNeasy membrane in the presence of high concentrations of chaotropic salt, which remove water from hydrated molecules in solution. Buffer conditions in DNeasy Blood & Tissue procedures are designed to enable specific adsorption of DNA to the silica membrane and optimal removal of contaminants and enzyme inhibitors.
Purification requires no phenol or chloroform extraction or alcohol precipitation, and involves minimal handling. This makes DNeasy Blood & Tissue Kits highly suited for simultaneous processing of multiple samples. For higher-throughput applications, the DNeasy 96 Blood & Tissue Kit enables simultaneous processing of 96 or 192 samples.
Reliable silica-membrane technology, in convenient spin-column or 96-well formats, ensures fast and reproducible DNA purification, eliminating the need for organic extraction and alcohol precipitation (see flowchart DNeasy Mini and 96 procedures). Samples are first lysed using proteinase K. Buffering conditions are adjusted to provide optimal DNA-binding conditions and the lysate is loaded onto the DNeasy Mini spin column or the DNeasy 96 plate. During centrifugation, DNA is selectively bound to the DNeasy membrane as contaminants pass through. Remaining contaminants and enzyme inhibitors are removed in two efficient wash steps and DNA is then eluted in water or buffer, ready for use.
DNeasy Mini spin columns are prepackaged in collection tubes and individually sealed, providing convenience and safety. The purification procedure using DNeasy Mini spin columns can be automated on the QIAcube. The DNeasy 96 Blood & Tissue Kit provides high-throughput processing in a 96-well format using the QIAGEN 96-Well-Plate Centrifugation System.
DNeasy Blood & Tissue Kits provide high-quality DNA, ready to use in all downstream assays, including applications in:
Life science research
Veterinary pathogen research
Routine applied testing
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