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MACHEREY NAGEL NucleoSpin RNA


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 Rp. 621,435
Katalog SL-740955.10
Brand Macherey Nagel
Kondisi New
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Harga dan Ketersediaan

Katalog SL-740955.10
Ukuran -
Ketersedian Tersedia    1
Harga Rp. 621,435
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NucleoSpin® RNA

RNA from cells and tissue

  Previously known as NucleoSpin® RNA II
Features 
 
Mini spin kit for the isolation of RNA of highest integrity

• rDNase included for on-column DNA digestion– easy and efficient removal of contaminating DNA
• NucleoSpin® Filters (shredders) – efficient sample homogenization and reduction of viscosity
• Up to 70 μg ready-to-use RNA
• Parallel purification of genomic DNA by using the NucleoSpin® RNA/DNA Buffer Set
   
Technology Silica-membrane technology
Format Mini spin columns
Sample material

< 5 x 106 cultured cells, 
< 109 bacterial cells, 
< 108 yeast cells, 
< 30 mg human / animal tissue
Fragment size > 200 nt
Typical yield

14 μg (106 HeLa cells), 70 μg (109 bacterial cells)
A260/A280 1.9–2.1
Typical RIN (RNA integrity number) > 9
Elution volume 40–120 µL
Preparation time 35 min/6 preps
Binding capacity 200 µg
 

Applications*
 
• RNA isolation from cultured cells, tissue (standard protocol)
• RNA from < 109 bacterial cells (Gram-negative, Gram-positive) or < 108 yeast cells
• RNA from ≤ 100 μL biological fluids
• RNA clean-up from reaction mixtures
• RNA from samples stored in RNAlater®
• RNA from saliva samples collected with Oragene®•RNA (Genotek)
• Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, 
  array technology, RNase protection assays
 

* Kits to be used for research purposes only

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