Monoclonal Mouse Anti-Human

Topoisomerase IIα

Clone Ki-S1

The topoisomerase II enzymes control DNA topology by cleaving and rejoining DNA strands and passing other DNA strands through the transient gaps. The topoisomerase IIα isoform is a 170 kDa nuclear protein that is only expressed in proliferating cells. Furthermore, topoisomerase IIα is an important cellular target of several chemotherapeutic drugs.

Isotype IgG2a, kappa

• Frozen
• Formalin
• HIER

 

Concentrated Antibody for Manual Use

• Excellent specificity
• High lot to lot consistency
• Optimized for immunohistochemistry (IHC) with validated protocols
• Certified manufacturing facilities guarantee full quality control

Dako's products are the result of almost 50 years experience ensuring quality and innovation in antibodies and reagents: the genesis of the pathology lab of tomorrow.

Intended Use

For in vitro diagnostic use.

Monoclonal Mouse Anti-Human Topoisomerase IIα, Clone Ki-S1, is intended for use in immunohistochemistry. The antibody labels topoisomerase IIα and is a useful tool for the identification of proliferating cells in normal tissues and in a number of human cancers (1-4). Differential identification is aided by the results from a panel of antibodies. The clinical interpretation of any staining or its absence should be complemented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

 

Immunogen

Lysate of the human histiocytic lymphoma cell line U-937 (1).

 

Specificity

In Western blotting of extract of yeast strain BJ 201 transfected with plasmid pHT 300α containing the human topoisomerase IIα gene, the antibody labels a band of 170 kDa corresponding to human topoisomerase IIα. No labelling was observed with extracts of yeast strain BJ 201 expressing S. pombe topoisomerase ll (7).

In Western blotting of human HL-60 cell lysates containing equal amounts of topo llα and topo llβ, the antibody only labels a 170 kDa band corresponding to the α isoform. The epitope recognized was found to be within the last 495, and most likely within the last 29, carboxyl-terminal amino acid residues of the protein (7).

 

Reagent Provided

Monoclonal mouse antibody provided in liquid form as purified IgG from ascitic fluid in 0.05 mol/L Tris/HCl, 15 mmol/L NaN3, 1% bovine serum albumin, pH 7.2.

Clone: Ki-S1 (1). Isotype: IgG2a, kappa.

Mouse IgG concentration: See label on vial.

 

References

1. Kreipe H, Heidebrecht HJ, Hansen S, Röhlk W, Kubbies M, Wacker HH, et al. A new proliferation-associated nuclear antigen detectable in paraffin-embedded tissues by the monoclonal antibody Ki-S1. Am J Pathol 1993;142:3-9.
2. Rudolph P, Lappe T, Schubert C, Schmidt D, Parwaresch RM, Christophers E. Diagnostic assessment of two novel proliferation-specific antigens in benign and malignant melanocytic lesions. Am J Pathol 1995; 147:1615-25.
3. Kreipe H, Alm P, Olsson H, Hauberg M, Fischer L, Parwaresch R. Prognostic significance of a formalin-resistant nuclear proliferation antigen in mammary carcinomas as determined by the monoclonal antibody Ki-S1. Am J Pathol 1993; 142:651-7.
4. Claviez A, Tiemann M, Peters J, Kreipe H, Schneppenheim R, Parwaresch R. The impact of EBV, proliferation rate, and Bcl-2 expression in Hodgkin's disease in childhood. Ann Hematol 1994; 68:61-6.

1. Boege F, Andersen A, Jensen S, Zeidler R, Kreipe H. Proliferation-associated nuclear antigen Ki-S1 is identical with topoisomerase IIαAm J Pathol 1995; 146:1302-8.

 

	Product	Size
	M7186 Purified : Specification Sheet Safety Data Sheet:Non-hazard classification statement	1 mL Product No. M718601

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