ELABSCIENCE Rat cTn I per TNNI3 (Cardiac Troponin I) ELISA KIT

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Katalog E-EL-R1253
Brand Elabscience
Kondisi New

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Katalog E-EL-R1253
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Rat cTn I per TNNI3 (Cardiac Troponin I) ELISA KIT Elabscience

Principle :

This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to cTn-I. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for cTn-I and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain cTn-I, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of cTn-I. You can calculate the concentration of cTn-I in the samples by comparing the OD of the samples to the standard curve.

Description :

Full Name   Rat cTn-I/TNNI3 (Cardiac Troponin I) ELISA Kit
  Product ID   E-EL-R1253   Organism   Rat
  Package   96T   Price   Inquire
  Detection Range   31.25~2000pg/mL   Sensitivity   18.75pg/mL


Components :

Item   Specifications   Storage
  Micro ELISA Plate   8 wells ×12 strips   4?
  Reference Standard   2 vials   4?
  Reference Standard & Sample Diluent   1vial 20mL   4?
  Concentrated Biotinylated Detection Ab   1vial 120μL   4?
  Biotinytated Detection Ab Diluent   1vial 10mL   4?
  Concentrated HRP Conjugate   1vial 120μL   4?(shading light)
  HRP Conjugate Diluent   1vial 10mL   4?
  Concentrated Wash Buffer (25×)   1vial 30mL   4?
  Substrate Reagent   1vial 10mL   4?(shading light)
  Stop Solution   1vial 10mL   4?
  Plate Sealer   5 pieces  
  Manual   1 copy  
  Desiccant   1 pack  
  Certificate of Analysis   1 copy


Procedure :

1. Add 100μL standard or sample to each well. Incubate 90 mintues at 37?

2. Add 100μL Biotinylated Detection Ab. Incubate 1 hour at 37?

3. Aspirate and wash 3 times

4. Add 100μL HRP Conjugate. Incubate 30 minutes at 37?

5. Aspirate and wash 5 times

6. Add 90μL Substrate Reagent. Incubate 15 minutes at 37?

7. Add 50μL Stop Solution. Read at 450nm immediately.

8. Calculation of results


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