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Rat CK MB (Creatine Kinase MB Isoenzyme) ELISA KIT Elabscience
This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CK-MB. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for CK-MB and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CK-MB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of CK-MB. You can calculate the concentration of CK-MB in the samples by comparing the OD of the samples to the standard curve.
|Full Name||Rat CK-MB (Creatine Kinase MB Isoenzyme) ELISA Kit|
|Micro ELISA Plate||8 wells ×12 strips||4?|
|Reference Standard||2 vials||4?|
|Reference Standard & Sample Diluent||1vial 20mL||4?|
|Concentrated Biotinylated Detection Ab||1vial 120μL||4?|
|Biotinytated Detection Ab Diluent||1vial 10mL||4?|
|Concentrated HRP Conjugate||1vial 120μL||4?(shading light)|
|HRP Conjugate Diluent||1vial 10mL||4?|
|Concentrated Wash Buffer (25×)||1vial 30mL||4?|
|Substrate Reagent||1vial 10mL||4?(shading light)|
|Stop Solution||1vial 10mL||4?|
|Plate Sealer||5 pieces|
|Certificate of Analysis||1 copy|
1. Add 100μL standard or sample to each well. Incubate 90 mintues at 37?
2. Add 100μL Biotinylated Detection Ab. Incubate 1 hour at 37?
3. Aspirate and wash 3 times
4. Add 100μL HRP Conjugate. Incubate 30 minutes at 37?
5. Aspirate and wash 5 times
6. Add 90μL Substrate Reagent. Incubate 15 minutes at 37?
7. Add 50μL Stop Solution. Read at 450nm immediately.
8. Calculation of results
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