T4 DNA Ligase catalyzes the formation of a phosphodiester bond between 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA. This enzyme will ligate DNA from blunt-end and cohesive-end termini (Including sticky ends for TA Cloning) as well as repair single stranded nicks in duplex DNA, RNA, or DNA/RNA hybrids. T4 DNA Ligase is isolated from a recombinant E.coli strain.
Features and Benefits
|High Speed:||DNA ligations in 5 minutes for cohesive end and in 10 minutes for blunt end DNA at 25°C|
|Flexibility:||Suitable for all common DNA ligations|
|Reproducibility:||Batch manufacturing under strict ISO 9001 quality control system|
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