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Hepatitis B virus infection can be determined by detecting HBsAg (HBV surface antigen). But in order to distinguish between chronic and active infection, one must quantify circulating HBV DNA levels and liver enzyme levels. Quantification through real-time PCR, which has superior sensitivity and specificity, allows physicians to validate treatment efficacy and offer more effective treatment.
The AccuPower® HBV Quantitative PCR Kit is an in vitro diagnostic kit designed for the quantification of hepatitis B virus (HBV) DNA in human samples such as EDTA-plasma through real-time PCR using ExiStation™ Molecular Diagnostic System or Exicycler™ 96.
Features and Benefits
|HBV Genotype Detection:||HBV genotype A, B, C, D, E, F and H are detectable with high sensitivity.|
|Signature Convenience:||All components for the assay are contained within a tube. Just add sample!|
| Enhanced Sensitivity & |
|Bioneer’s proprietary HotStart technology accomplishes high sensitivity and specificity.|
|Remarkable Stability:||Vacuum-dried premix allows stable and reproducible results.|
|High Quality:||All diagnostic kits manufactured by Bioneer are under strict quality control guidelines.|
|Target||Hepatitis B virus (large S protein)|
|Sensitivity||LoD: 9.55 IU/ml (genotype A2), LoQ: 50 IU/ml|
|Kit Contents||PCR Premix, SPC 1~5, IPC, NTC, SL buffer, DEPC D.W.|
|Instrumentation||ExiStation™ Universal Molecular Diagnostic System or Exicycler™ 96|
Fig 1. Standard Positive Control (flu. graph)
Fig 2. Standard Curve
AccuPower® HBV Quantitative PCR Kit includes serially diluted Standard Positive Control (SPC) 1~5 for the quantification of HBV.
Fig 3. Positive Sample (flu. graph)
Fig 4. Negative Sample (flu. graph)
AccuPower® HBV Quantitative PCR Kit test results using clinical samples. The kit employs IPC in all wells to confirm correct PCR amplification.
Fig 5. Final Results
User centric ExiStation Manager software or ExiDiagnosis software automatically analyzes the test results based upon the Ct (threshold cycle) value.
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