BIONEER AccuPower MTB dan NTM Real Time PCR Kit 96 T


Mulai dari

Rp. 18,000,000
Katalog MTN-1111
Brand Bioneer
Kondisi New
Lampiran

Harga dan Ketersediaan

Katalog MTN-1111
Ukuran 96 T
Ketersedian Tersedia    2
Harga Rp. 18,000,000
Ulasan

Tuberculosis (TB) is caused by a bacterium called M. tuberculosis. The bacteria usually attack the lungs, but TB bacteria can attack any part of the body such as the kidney, spine, and brain. It can affect mainly the lungs (pulmonary TB). Non-tuberculous mycobacteria (NTM) are a group of bacteria, normally found in soil and water and some domestic and wild animals that can cause severe lung disease.

 

Diagnosing MTB, NTM can be difficult because symptoms may be similar to other lung conditions. Recently, molecular diagnostic methods, including PCR or real-time PCR, provide higher sensitivity and specificity than other conventional diagnostic methods.

Intended Use

The AccuPower® MTB&NTM Real-Time PCR Kit is an in vitro diagnostic kit designed for the simultaneous detection of Mycobacterium tuberculosis (MTB) and non-tuberculous mycobacteria (NTM) DNA in human samples such as sputum, bronchoalveolar lavage (BAL) and urine through real-time PCR using ExiStation™ Molecular Diagnostic System or Exicycler™ 96.

Features and Benefits

 Signature Convenience: Simultaneous detection of MTB and NTM (57 species)
Simplified procedure of sputum pretreatment with EZ Solution
 Enhanced Sensitivity &
   Specificity:
Bioneer’s proprietary HotStart technology accomplishes high sensitivity and specificity.
 Remarkable Stability: Vacuum-dried premix allows stable and reproducible results.
 High Quality: All diagnostic kits manufactured by Bioneer are under strict quality control guidelines.

Specifications

  Target Mycobacteria tuberculosis (IS6110)
Non-tuberculous mycobacteria (rpoB)
  Specimen Type Sputum, bronchoalveolar lavage (BAL), urine
  Sensitivity (LoD) MTB: 20.4 copies/test
NTM (M. avium 44.7 copies/test, M. intracellulare 158.5 copies/test, M. abscessus 72.4 copies/test, M.chelonae 52.5 copies/test, M. kansasii 190.5 copies/test, M. fortuitum 53.7 copies/test, M. godonae 109.6 copies/test, M. massilliense 57.5 copies/test)
  Kit Contents PCR Premix, PC, IPC, NTC, DEPC D.W.
  Instrumentation ExiStation™ ™ Universal Molecular Diagnostic System or Exicycler™ 96
  Tests 96



 

Experimental Data

AccuPower MTB&NTM PC graph
Fig 1. Positive Control (flu. graph)
AccuPower MTB&NTM NTC graph
Fig 2. No Template Control (flu. graph)

 

AccuPower® MTB&NTM Real-Time PCR Kit includes Positive Control (PC), No Template Control (NTC) and Internal Positive Control (IPC) for accurate and reliable diagnosis of MTB and NTM.

 

AccuPower MTB positive sample graph
Fig 3. MTB Positive Sample (flu. graph)
AccuPower NTM positive sample graph
Fig 4. NTM Positive Sample (flu. graph)

 

AccuPower® MTB&NTM Real-Time PCR Kit test results using clinical samples. The kit employs IPC in all wells to confirm correct PCR amplification.

 

AccuPower MTB&NTM final result
Fig 5. Final Results

 

User centric ExiStation Manager software or ExiDiagnosis software automatically analyzes the test results based upon the Ct (threshold cycle) value.

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