BIONEER AccuPower BCR ABL Quantitative RT PCR Kit 96 T


Mulai dari

Rp. 25,800,000
Katalog CML-1111
Brand Bioneer
Kondisi New
Lampiran

Harga dan Ketersediaan

Katalog CML-1111
Ukuran 96 T
Ketersedian Tersedia    2
Harga Rp. 25,800,000
Ulasan

 

Philadelphia chromosome or Philadelphia translocation is a specific chromosomal abnormality that is associated with chronic myelogenous leukemia (CML). It is the result of a reciprocal translocation between chromosome 9 (region q34: ABL) and 22 (region q11: BCR). ABL gene expresses a membrane-associated protein, a tyrosine kinase, the BCR-ABL transcript is also translated into a tyrosine kinase, adding a phosphate group to tyrosine. In turn, BCR-ABL activates a number of cell cycle-controlling proteins and enzymes, speeding up cell division. Moreover, it inhibits DNA repair, causing genomic instability and potentially causing the feared blast crisis in CML.

 

Intended Use

The AccuPower® BCR-ABL Quantitative RT-PCR Kit is an in vitro diagnostic kit designed for the quantification of BCR-ABL (M-bcr) mutation in human samples such as whole blood and bone marrow through real-time PCR using Exicycler™ 96.

Features and Benefits

 Signature Convenience: One-step RT-PCR premix type.
All components for the assay are contained within a tube. Just add sample!
 Enhanced Sensitivity &
   Specificity:
Bioneer’s proprietary Dual HotStart technology accomplishes high sensitivity and specificity.
 Remarkable Stability: Vacuum-dried premix allows stable and reproducible results.
 High Quality: All diagnostic kits manufactured by Bioneer are under strict quality control guidelines.

 

Specifications

  Target ABL (reference gene), BCR-ABL (M-bcr: b3a2, b2a2)
  Specimen Type Whole blood, bone marrow
  Kit Contents BCR-ABL PCR Premix, ABL PCR Premix, SPC 1~5
  Instrumentation Exicycler™ 96
  Tests 48


 

Experimental Data

AccuPower CML ABL SPC graph
Fig 1. ABL Standard Positive Control (flu. graph)
AccuPower CML M-bcr SPC graph
Fig 2. M-bcr Standard Positive Control (flu. graph)

 

AccuPower® BCR-ABL Quantitative RT-PCR Kit includes serially diluted Standard Positive Control (SPC) 1~5 for the quantification of BCR-ABL (M-bcr) mutation.

 

AccuPower CML M-bcr positive sample
Fig 3. M-bcr Positive Sample (flu. graph)
AccuPower CML M-bcr negative sample
Fig 4. M-bcr Negative Sample (flu. graph)

 

AccuPower® BCR-ABL Quantitative RT-PCR Kit test results using clinical samples.

 

AccuPower CML analytical sensitivity
Fig 5. Analytical sensitivity of AccuPower® BCR-ABL Quantitative RT-PCR Kit

 

Correlation of the M-bcr mutation ratio analyzed by AccuPower® BCR-ABL Quantitative RT-PCR Kit and the known M-bcr mutation ratio (K-562/normal cell total RNA mix test)

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