| Rp. 25,800,000 |
Philadelphia chromosome or Philadelphia translocation is a specific chromosomal abnormality that is associated with chronic myelogenous leukemia (CML). It is the result of a reciprocal translocation between chromosome 9 (region q34: ABL) and 22 (region q11: BCR). ABL gene expresses a membrane-associated protein, a tyrosine kinase, the BCR-ABL transcript is also translated into a tyrosine kinase, adding a phosphate group to tyrosine. In turn, BCR-ABL activates a number of cell cycle-controlling proteins and enzymes, speeding up cell division. Moreover, it inhibits DNA repair, causing genomic instability and potentially causing the feared blast crisis in CML.
The AccuPower® BCR-ABL Quantitative RT-PCR Kit is an in vitro diagnostic kit designed for the quantification of BCR-ABL (M-bcr) mutation in human samples such as whole blood and bone marrow through real-time PCR using Exicycler™ 96.
Features and Benefits
|Signature Convenience:||One-step RT-PCR premix type. |
All components for the assay are contained within a tube. Just add sample!
| Enhanced Sensitivity & |
|Bioneer’s proprietary Dual HotStart technology accomplishes high sensitivity and specificity.|
|Remarkable Stability:||Vacuum-dried premix allows stable and reproducible results.|
|High Quality:||All diagnostic kits manufactured by Bioneer are under strict quality control guidelines.|
|Target||ABL (reference gene), BCR-ABL (M-bcr: b3a2, b2a2)|
|Specimen Type||Whole blood, bone marrow|
|Kit Contents||BCR-ABL PCR Premix, ABL PCR Premix, SPC 1~5|
Fig 1. ABL Standard Positive Control (flu. graph)
Fig 2. M-bcr Standard Positive Control (flu. graph)
AccuPower® BCR-ABL Quantitative RT-PCR Kit includes serially diluted Standard Positive Control (SPC) 1~5 for the quantification of BCR-ABL (M-bcr) mutation.
Fig 3. M-bcr Positive Sample (flu. graph)
Fig 4. M-bcr Negative Sample (flu. graph)
AccuPower® BCR-ABL Quantitative RT-PCR Kit test results using clinical samples.
Fig 5. Analytical sensitivity of AccuPower® BCR-ABL Quantitative RT-PCR Kit
Correlation of the M-bcr mutation ratio analyzed by AccuPower® BCR-ABL Quantitative RT-PCR Kit and the known M-bcr mutation ratio (K-562/normal cell total RNA mix test)
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