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MACHEREY NAGEL NucleoSpin Gel and PCR Clean Up (50 preps)


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 Rp. 1,009,687
Rp. 3,365,623
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Katalog 740609.5
Brand Macherey Nagel
Kondisi New
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Katalog 740609.5
Ukuran -
Ketersedian Hubungi Kami
Harga Rp. 1,009,687
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NucleoSpin Gel and PCR Clean-up

Features

PCR clean-up and gel extraction the two in one kit with optimized recovery and elution volume

• Two applications in one kit – one buffer with optimal performance for both applications
• High recoveries for fragments down to 50 bp
• Minimized elution volume of 15 μL – highly concentrated DNA
• Binding buffer with pH indicator
• Separate buffers for single stranded DNA or SDS containing samples available
• Suitable for all gel buffer systems (e.g., TAE, TBE)
 

Technology Silica-membrane technology
Format Mini spin columns
Sample material

< 400 µL PCR reaction mixture 
< 400 mg TAE / TBE agarose gel
Fragment size 50 bp–approx. 20 kbp
Typical recovery 70–95%
A260/A280 1.8–1.9
Elution volume 15–30 µL
Preparation time 10 min/6 preps
Binding capacity 25 µg

Applications

• Purification of PCR products
• Extraction of DNA from agarose gels
• DNA extraction from polyacrylamide gels
• Clean-up of single stranded DNA and RNA extraction from agarose gels (Binding Buffer NTC required, not included in the kit, see “Ordering information”)
• Clean-up of SDS-containing samples (Binding Buffer NTB required, not included in the
   kit, see “Ordering information”)
• Typical downstream applications: cloning, sequencing, PCR, restriction analysis
• NucleoSpin Gel and PCR Clean-up ensures complete removal of contaminations such as:
- nucleotides, primers
- enzymes
- mineral oil
- PCR additives (e.g., salts, betaine, DMSO)
- detergents (e.g., Tween 20, Triton X-100)
- dyes (e.g., ethidiumbromide, crystal violet, Stain G, Midori Green, Roti-Safe GelStain, DNA SafeStain)

 

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