Description:

NAGEL NucleoSpin RNA (50)

Technology: Silica-membrane technologyFormat: Mini spin columnsSample material: < 5 x 106 cultured cells, < 109 bacterial cells, < 108 yeast cells, < 30 mg human / animal tissueFragment size: > 200 ntTypical yield: 14 μg (106 HeLa cells), 70 μg (109 bacterial cells)A260/A280: 1.9–2.1Typical RIN (RNA integrity number): > 9Elution volume: 40–120 µLPreparation time: 35 min/6 prepsBinding capacity: 200 µg

Applications*

• RNA isolation from cultured cells, tissue (standard protocol)• RNA from < 109 bacterial cells (Gram-negative, Gram-positive) or < 108 yeast cells
• RNA from ≤ 100 μL biological fluids
• RNA clean-up from reaction mixtures
• RNA from samples stored in RNAlater®
• RNA from saliva samples collected with Oragene®•RNA (Genotek)
• Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension,
  array technology, RNase protection assays