NEXpro ™ HS Taq DNA Polymerase is a DNA polymerase that inhibits DNA polymerase activity and 3 '? 5' exonuclease activity at room temperature. By adding clonal antibody, it is possible to reduce nonspecific phenomenon such as non-specific, primer dimer which occurs before PCR reaction before reaching high temperature. It is a DNA polymerase suitable for hot real-time PCR and multiplex PCR based on high accuracy and amplification efficiency. Application : ? Amplification of DNA fragments shorter than 3 kb ? Hot-start PCR ? cDNA / genomic DNA amplification ? Template amplification of a higher structure that is difficult to amplify ? Primer extension ? Multiplex PCR Source : Recombinant proteins expressed in E. coli. Characteristics : ? Error rate : 2.4 × 10-5 ? Thermal stability : Half-life of 40 mins at 95? ? A-tail formation at 3’ends of amplified duplex DNA ? Primer-dimer formation / non-specific amplification reduction ? RT setup possible ? Antibody based hot-start PCR Free of non-specific nuclease Storage : -20 ? recommended Concentration : 5 Unit / ?
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