The ProPac IMAC-10 22X250MM is an analytical HPLC column packed with 10 Âµm, nonporous, polymeric beads that are coated with a hydrophilic layer, then grafted with poly(IDA) chains. The poly(IDA) grafts are converted to metal-containing nanoparticles when the column is charged with metal. It is these nanoparticles that act as IMAC interaction sites for individual proteins and provide the ProPac IMAC-10 with its high resolving power. â€¢ State-of-the-art technology for reusable columns with metal tailored specificity â€¢ Resolve target proteins using a single column in a high resolution gradient run â€¢ Retention control by imidazole concentration or pH gradient â€¢ High purity separations of metal-binding proteins â€¢ Wide range of metal-specific applications The ProPac IMAC-10 design maximizes the number of sterically accessible ligands for binding to proteins, ensuring that the proteins remain tightly bound during separation. It can be operated between pH 2â€“12 and is compatible with commonly used protein purification reagents (non-ionic detergents, reducing agents). It can be used under native or denaturing HPLC conditions. The ProPac IMAC-10 is shipped free from any metal and ready to be charged with the metal of your choice. To avoid contact of the metal solution with your system, charge the IMAC-10 with a loading column (an empty column body you fill with your metal solution, then place between the pump and the IMAC-10 column).