Resolve target proteins using a single column in a high-resolution gradient run with Thermo Scientificâ„¢ ProPacâ„¢ IMAC-10 LC Columns. These analytical and semi-preparative HPLC columns provides high-purity separations of metal-binding proteins and high loading capacity for protein purification applications. The use of small, nonporous resin particles results in narrow, high-efficiency peaks, with minimal band broadening due to dispersion. Use loading columns to charge the ProPac IMAC-10 LC columns but avoid contact of the metal solution with the system. ProPac IMAC-10 Load 9X50MM.