Acclaim Mixed-Mode HILIC-1 Uniquely designed for both reversed-phase and HILIC operations â€¢ Can operate in both RP and HILIC modes â€¢ Retains highly polar molecules â€¢ Unique selectivity complementary to RP columns â€¢ Broader application range compared with conventional diol-based columns â€¢ High-efficiency column for high-resolution separations The Acclaim Mixed-Mode HILIC-1 column features a unique, high-efficiency, silica-based HPLC mixed-mode stationary phase that combines both reversed-phase (RP) and hydrophilic interaction liquid chromatography (HILIC) properties. This combination allows both hydrophobic and hydrophilic interactions to be utilized to optimize separations. The functional group is of a hydrophobic alkyl chain with a diol group at the terminus. This unique combination results in the adjustable selectivity, making Acclaim Mixed-Mode HILIC-1 separate mixtures that would be impossible for a C18 column. This column is suitable for a broad range of applications, including non-ionic ethoxylated surfactants, drug metabolites, lipids, polyethylene glycols (PEGs), ethoxylated surfactants, and more.